My primary research focus is the development of novel methods to guide cell engineering in Chinese hamster ovary (CHO) cells to produce better therapeutics for human diseases. A secondary research focus is the application of systems biology methods to study the impacts of glycosylation alterations in CHO cells.
- Towards a virus-resistant mammalian cell
Virus contamination is an important but unresolved issue in manufacturing biopharmaceutical products. This work applied RNA-seq analyses to elucidate the pathways that are significantly altered upon viral infection of CHO cells. Moreover, the identification of genes associated with the virus-susceptible phenotypes will allow us to develop interventions to improve the success of virus-resistant CHO efforts.
- Systematic analysis of the impact of glycoengineering in CHO cells
Diverse engineered glycosylation can affect the recombinant protein efficacy and also influence many cell pathways and consequently modulating disease physiologies. By applying innovative systems biology approaches, we aim to provide insights into how critical metabolic and signaling pathways are modulated by alterations in glycosylation. Finally, an integrated knowledge about the pathways by which changes in glycosylation can lead to disease will be revealed.
Link to my Google Scholar
Email Address: w3chiang (at) ucsd (dot) edu